A.Y. 2020-21

Developmental Biology of Higher organisms

This course contains following subjects for the semester end examination December 2020:

1.      History of computation biology                        (2 Lecture)

2.     Introduction of computation biology packages (6 Lecture)

2.1.   Python and biopython

2.2.   Perl and biolperl

2.3.   R packages, bioconductor

2.4.   MySql

2.5.   MATLAB

3.     Application of computation biology in research and development (2)

4.     Current status and future prospects of computational biology (5)

5.     Hands on and the tutorials (5 Practicals)

5.1.   Python and biopython

5.2.   Perl and biolperl

5.3.   R package

5.4.   MySql

5.5.   MATLAB

This is a course on Microbial Biotechnology with respect to environmental and agricultural applications. 

This is a course on Biodiversity 

This is an internal exam.

Instructions:

1) Each question carries 2 marks

2) Time duration is 30 minutes

3) Total marks are 20

4) You are expected to attempt a minimum of 10 questions.

5) Test starts at 12 noon

6) Test closes at 2pm. But, you will get only 30 min to attempt the test once you start attempting.

7) Click submit all and finish option twice to make sure that your attempts are successfully submitted for review.

Understanding genomes

General concepts in Genetics

Plant Genetics

Animal Genetics

1. Process calculations and stoichiometry                                                       3h 

2. Metabolic engineering                                                                                      2h

3. Transport in reactors (oxygen, substrates, heat, (material balance)    7h

4. Bioreactor design

Types of reactors, sterilization Utilities: steam air water                                  7h

Solid-state fermentation                                                                                         2h

Biotransformations                                                                                                    2h

Instrumentation and control (probes of different types)                                 2h

Specific industrial process applications in plant systems                               7h

(Suspension callus and hairy root cultures)

.ii) Specific industrial processes involving microbes                                         4h

Specific industrial process applications in animal systems

Background, need, current products, cell lines.                                                   1h

Production strategy, Production platform                                                             1h

Vector design                                                                                                               1h

Cell line development                                                                                                2h

Media development                                                                                                   2h

Bioreactor design (animal cell culture specific)                                                  1 h

Downstream formulation packaging                                                                      1 h

The Animal Cell Culture course deals with detailed study of procedures, techniques and instruments utilized for handling and culturing cells from animal origin. 

1. Nutrition in tissue culture - Balanced salt solution, synthetic media, sera, dissociation media, antibiotics, growth factors and substrates 2L

2. Concept of sterilization and aseptic technique, Sources of contamination in tissue culture and their its prevention 2L

3. Cryopreservation of cell 2L

4. Types of cells in tissue culture- epithelial cells, fibroblast, Mass culture of cells for production 2L

5. Classification: anchorage dependant and independent, stem cells, fastidious cell culture, kerotinocytes, melanocytes and nerve cells 2L

6. Primary culture, diploid culture and established cell lines and characterization 2L

7. Specialized cell culture : for biomedical application, hepatocytes, islets, skin cells, vascular endothelial cells 2L

8. Primary culture, diploid culture and established cell line and characterization 2L

9. Cell growth curve, viability, MTT assays 2L

10. FACS, confocal , immunoflourescence, thymidine uptake cytotoxicity 2L

11. Cell fusion 2L

12. Types of stem cells and their use in tissue engineering 2L

13. Tumerogenesis , angiogenesis metastatis in in vivo and in vitro studies

14. Application of organ culture in virology and toxicology 2L

15. Cytogenetics studies, chromosome preparation and banding techniques 2L

16. Principles of cell separation and purification of cells and their products 2L

1. Metabolism overview 

2. Glycolysis 

3. Fermentation, regulation of glycolysis, and the pentose phosphate pathway 

4. Pyruvate oxidation and the citric acid cycle 

5. Oxidative phosphorylation and Electron transfers in biology 

6. ATP and phosphoryl group transfers 

7. Gluconeogenesis, Glycogen metabolism and Anaplerotic reactions

8. Photosynthesis, photophosphorylation and transpiration 

9. Fatty acid oxidation 

10. Fatty acid,lipid / phospholipid and sterol synthesis 

11. Aminoacid metabolism 

12. Nitrogen metabolism .heme metabolism and urea cycle

13. Nucleotide synthesis and degradation 

14. Diseases of Protein and Nucleic Acid Breakdown 

15. Integration of central metabolic pathway