
Semester IX Term End Examination December 2020
This course contains following subjects for the semester end examination December 2020:
Subject / Course Code |
Subject / Course Name |
IBT- 605 T+P |
Understanding Genomes |
IBT-701 T |
Fermentation Technology Downstrream processing |
IBT 702T |
Applications of Plant Biotech in Agriculture |
IBT 703T |
Vaccines |
IBT 801 T |
IPR |

Introduction to Computation Biology
1. History of computation biology (2 Lecture)
2. Introduction of computation biology packages (6 Lecture)
2.1. Python and biopython
2.2. Perl and biolperl
2.3. R packages, bioconductor
2.4. MySql
2.5. MATLAB
3. Application of computation biology in research and development (2)
4. Current status and future prospects of computational biology (5)
5. Hands on and the tutorials (5 Practicals)
5.1. Python and biopython
5.2. Perl and biolperl
5.3. R package
5.4. MySql
5.5. MATLAB

Sem V Microbial Biotechnology I and II
This is a course on Microbial Biotechnology with respect to environmental and agricultural applications.

Introduction to Analytical Techniques and Applications
Theory Module |
Lectures |
1. Homogenization and Sonication techniques |
2 |
2. Filtration and dialysis techniques |
3 |
3. Centrifugation techniques |
2 |
4. Viscosity |
1 |
5. Electrochemical cell, pH and electrodes |
2 |
6. Chromatography principles: Paper, TLC, Gel filtration, affinity chromatography, Adsorption chromatography |
5 |

Sem III C programming
This is an internal exam.
Instructions:
1) Each question carries 2 marks
2) Time duration is 30 minutes
3) Total marks are 20
4) You are expected to attempt a minimum of 10 questions.
5) Test starts at 12 noon
6) Test closes at 2pm. But, you will get only 30 min to attempt the test once you start attempting.
7) Click submit all and finish option twice to make sure that your attempts are successfully submitted for review.

Sem V IBT-404T Genetics of higher organisms
General concepts in Genetics
Plant Genetics
Animal Genetics

Sem IX Fermentation Technology and Down Stream Processing/ Cybersecurity
1. Process calculations and stoichiometry 3h
2. Metabolic engineering 2h
3. Transport in reactors (oxygen, substrates, heat, (material balance) 7h
4. Bioreactor design
Types of reactors, sterilization Utilities: steam air water 7h
Solid-state fermentation 2h
Biotransformations 2h
Instrumentation and control (probes of different types) 2h
Specific industrial process applications in plant systems 7h
(Suspension callus and hairy root cultures)
.ii) Specific industrial processes involving microbes 4h
Specific industrial process applications in animal systems
Background, need, current products, cell lines. 1h
Production strategy, Production platform 1h
Vector design 1h
Cell line development 2h
Media development 2h
Bioreactor design (animal cell culture specific) 1 h
Downstream formulation packaging 1 h

Sem VII (IBT311T) Introduction to Animal Tissue Culture
The Animal Cell Culture course deals with detailed study of procedures, techniques and instruments utilized for handling and culturing cells from animal origin.
1. Nutrition in tissue culture - Balanced salt solution, synthetic media, sera, dissociation media, antibiotics, growth factors and substrates 2L
2. Concept of sterilization and aseptic technique, Sources of contamination in tissue culture and their its prevention 2L
3. Cryopreservation of cell 2L
4. Types of cells in tissue culture- epithelial cells, fibroblast, Mass culture of cells for production 2L
5. Classification: anchorage dependant and independent, stem cells, fastidious cell culture, kerotinocytes, melanocytes and nerve cells 2L
6. Primary culture, diploid culture and established cell lines and characterization 2L
7. Specialized cell culture : for biomedical application, hepatocytes, islets, skin cells, vascular endothelial cells 2L
8. Primary culture, diploid culture and established cell line and characterization 2L
9. Cell growth curve, viability, MTT assays 2L
10. FACS, confocal , immunoflourescence, thymidine uptake cytotoxicity 2L
11. Cell fusion 2L
12. Types of stem cells and their use in tissue engineering 2L
13. Tumerogenesis , angiogenesis metastatis in in vivo and in vitro studies
14. Application of organ culture in virology and toxicology 2L
15. Cytogenetics studies, chromosome preparation and banding techniques 2L
16. Principles of cell separation and purification of cells and their products 2L

Metabolic Pathways
1. Metabolism overview
2. Glycolysis
3. Fermentation, regulation of glycolysis, and the pentose phosphate pathway
4. Pyruvate oxidation and the citric acid cycle
5. Oxidative phosphorylation and Electron transfers in biology
6. ATP and phosphoryl group transfers
7. Gluconeogenesis, Glycogen metabolism and Anaplerotic reactions
8. Photosynthesis, photophosphorylation and transpiration
9. Fatty acid oxidation
10. Fatty acid,lipid / phospholipid and sterol synthesis
12. Nitrogen metabolism .heme metabolism and urea cycle
13. Nucleotide synthesis and degradation
14. Diseases of Protein and Nucleic Acid Breakdown
15. Integration of central metabolic pathway

Basic Separation Techniques in Biology
1. HPLC, HPAEC, GC, Hydrophobic Interaction Chromatography, countercurrent chromatography |
2. Advanced techniques: LC-MS, GC, GC-MS, SEC-MALS |
3. Cell organelle separation/ Ultracentrifugation |
4. Labeling techniques |
5. Pull down assays |
6. Ultrafiltration |
7. Electrophoresis, Capillary electrophoresis, counter current immune-electrophoresis |