Biomolecules
SHADAB AHMEDAMEETA RAVIKUMAR

Biomolecules

1. Introduction /overview 2L

2. Water and Intermolecular Forces 2L

3. Thermodynamics in Biochemistry 2L

4. pH, acids bases and Buffers 3L

5. Amino Acids 2L

6. Peptide Bonds and Introduction to Proteins and Ramchandran plot 3L

7. Sugars and Polysaccharides 2L

8 Glycoproteins and Proteoglycans 1L

9 Lipids 2L

10 Membranes 2L

11 Membrane Proteins 1L

12 Nucleosides and Nucleotides 1L

13 Primary Structure of Nucleic Acids 5L

14 Vitamins, Coenzymes and other small molecules 2L

Enzyme Technology
SHADAB AHMEDAMEETA RAVIKUMAR

Enzyme Technology

1. Fundamentals of enzyme kinetics 3L

2. Enzyme preparation and use 3L

3 The preparation of immobilised enzymes – rationale ,

choice of matrix, methods of immobilization 5L

4. Large scale enzyme production 3L

5. Immobilised enzymes : kinetics and their uses 5L

6. Whole cell immobilization

7. Enzyme stabilization: use of additives 2L

8. Application of enzymes in industry, analytical purpose

and medical therapy. – case studies 5L

9 Biphasic systems 2L

10. Future prospects for enzyme technology 2L

Nucleic Acid and Protein Chemistry
SHADAB AHMEDAMUL SAKHARKAR

Nucleic Acid and Protein Chemistry

Nucleic acid chemistry                                                                                                           15L

1 DNA and RNA structure regular and irregular and dynamic structures

2  Chemical Synthesis of oligodeoxyribonucleotides Esters and anhydrides of Oxy phosphorus acids

-Preparation of monomers, protecting and deprotecting groups

-Diester triester and and H-phosphonate chemistry

-Solid-phase organic synthesis - phosphramidate chemistry

-Synthesis of oligoribonucleotides

-Synthesis of modified oligonuleotides and their applications

3. Sequencing methods

4. Biosynthesis of nucleic acids

Biosynthesis of purine and pyrimidines nucleotides (De novo and salvage pathways)

- Drug inhibition of biosynthesis

-Polymerization

-Applications of synthetic oligonucleotides

Molecular biology, diagnostics and medicine

Antisense and anti gene therapy

Protein chemistry 15L

1. Background information : Amino acids, peptide bonds,primary and secondary

structures ( 1 L).

Protein folding structure and function

Determination of primary structure............................: Techniques, interpretation etc.

including post translational modification of proteins( viz. glycosylation, N-terminal

modification, hydroxylation & modified amino acids) ( 2 L).

Determination of secondary structure...................UV, CD and fluorescence ( 2 L).

Determination of quaternary structure.................................: X-ray ( 2 L.)

Functional proteins ................: Hemoglobin and some well characterised

enzymes / lectins / peptide hormones ( 4 L).

3. Solid phase synthesis of peptides and their biological applications (2L)

4. Chemical modifications (2L)

Biochemical & Biophysical Techniques
SHADAB AHMEDBimalendu Nath

Biochemical & Biophysical Techniques

1.      Principles, Applications of UV-Visible, fluorescence (including FRET & Anisotropy), CD and ORD, ESR, Dynamic Light Scattering, Raman Spectroscopy, Mass spectroscopy

8 h

2.      Surface plasmon response (SPR)

1 h

  1. Isothermal titration calorimetry (ITC)

1 h

  1. Microscale thermophoresis

2 h

5.      Microscopy Techniques

10 h

6.      Principle and Application of Electrophoresis

3 h

7.      Advanced column chromatography  and Chromatographic calculations

4 h

8.      Surface tension and contact angle measurement

1 h


Plant Physiology
VAIJAYANTI TAMHANE

Plant Physiology

Plant mineral nutrition

Plant growth hormones

Plant specialized metabolism

Water relations

Phloem loading and source sink relations

Photosynthesis


Fundamentals of Immunology
Tuli Dey

Fundamentals of Immunology

1.   Overview of immunology

2.   Cell and organs of immune system

3.   Generation of B cells and T cells response

4.   Antibodies structure and function

5.   Organization and expression of immunoglobin gene

6.   Antigen and antibody interaction principles and application

7.   Major histocompatibility complex

8.   Antigen processing and presentation

9.   T cell receptor